Abstract:Elymus nutans, widely distributed in natural and cultivated pastures with favorable forage yield, good quality and adaptability to local environment, plays an important role in animal husbandry and environmental sustainability on Qinghai-Tibetan Plateau, China. Silicon (Si) is not considered as an essential element for higher plants and thus is believed to have no effect on primary metabolism in unstressed plants. But previous studies have shown that silicon can promote nutrient root absorption and enhance plant productivity in various plant species such as rice, alfalfa and celery. In greenhouse study, silicon nutrition improves root absorption of nitrogen and phosphorus; however, no attempt has been made to study the absorption of nitrogen and phosphorus in root and the content of nitrogen and phosphorus in leaf when silicon, nitrogen and phosphorus were added at different concentration in field. A study was conducted with Elymus nutans to address the physiological effect caused by the addition of these elements. Sixteen treatments consisted of: three rates of nitrogen addition: 7, 14, 21 g/m2; three rates of phosphorus addition: 4.92, 9.84, 14.76 g/m2; three rates of available silicon addition: 0.718, 1.436, 2.154 g/m2; three rates of nitrogen and one rate of silicon addition: nitrogen 7, 14, 21 g/m2 and silicon 1.436 g/m2; three rates of phosphorus and one rate of silicon addition: phosphorus 4.92, 9.84, 14.76 g/m2 and silicon 1.436 g/m2; and one control: neither nitrogen and phosphorus nor silicon addition. Each treatment was replicated six times. Results showed that in soil, nitrogen addition alone enhanced contents of NH4+-N (P<0.001) and NO3--N (P<0.001). Silicon addition alone or addition with phosphorus did not affect NH4+-N content. Phosphorus addition alone enhanced total and available phosphorus contents. Low concentration silicon addition alone did not affected NO3--N (P>0.5) content, or total and available phosphorus content (P>0.5). High concentration silicon addition alone, silicon addition with nitrogen or phosphorus together increased NO3--N content (P<0.001), or total and available phosphorus content (P<0.001). In leaf of E. nutans, nitrogen and phosphorus addition alone enhanced total nitrogen and phosphorus content. Low concentration silicon addition alone did not affect total nitrogen and phosphorus content. High and moderate concentration silicon addition alone, silicon addition with nitrogen or phosphorus together can increase leaf of total nitrogen and phosphorus content. There were significant linear positive relationships between total nitrogen content in leaf of E. nutans and soil NH4+-N (R2=0.96, P<0.001) and NO3--N content (R2=0.91, P<0.001) and also between leaf total phosphorus and soil total phosphorus (R2=0.88, P<0.001) and soil available phosphorus content (R2=0.96, P<0.001). In the field experiment, silicon can promote the uptake of nitrogen and phosphorus by E. nutans. These results suggested that silicon application is useful to increase leaf nitrogen and phosphorus content of E. nutans through the enhancement of NO3--N, total and available phosphorus content in soil. We identify Si nutrition as an important target in attempts to improve the uptake of nitrogen and phosphorus by E. nutans.