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宋玉芳,李昕馨,张薇,David Freestone,M. Leigh Ackland,陈朗,吉普辉,杨晓霞.植物CytP450和抗氧化酶对土壤低浓度菲、芘胁迫的响应.生态学报,2009,29(7):3768~ 本文二维码信息
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植物CytP450和抗氧化酶对土壤低浓度菲、芘胁迫的响应
The response of CytP450 and antioxidant enzymes in plants to Phenanthrene and Pyrene exposure in soil at lower concentrations
投稿时间:2008-04-05  修订日期:2008-10-20
DOI:
关键词土壤  植物  CytP450  抗氧化酶    
Key Wordssoil  plant  CytP450  antioxidant enzymes  phenanthrene  pyrene
基金项目国家重点基础研究发展规划973资助项目(2004CB418503);国家科技支撑计划资助项目(2007BAD89B03); 国家高技术研究发展863资助项目(2006AA06Z386)
作者单位E-mail
宋玉芳 中国科学院沈阳应用生态研究所沈阳 110016 Songyufang@iae.ac.cn 
李昕馨 沈阳大学环境工程重点实验室沈阳 110041  
张薇 沈阳农业大学土地与环境学院沈阳 110161  
David Freestone 澳大利亚Deakin大学细胞与分子生物学中心  
M. Leigh Ackland 澳大利亚Deakin大学细胞与分子生物学中心  
陈朗 中国科学院沈阳应用生态研究所沈阳 110016中国科学院研究生院北京 100039  
吉普辉 中国科学院沈阳应用生态研究所沈阳 110016中国科学院研究生院北京 100039  
杨晓霞 中国科学院沈阳应用生态研究所沈阳 110016中国科学院研究生院北京 100039  
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摘要:
以小麦(Triticum acstivnm)为供试植物,草甸棕壤为供试土壤,以微粒体细胞色素P450及抗氧化酶SOD、POD和CAT酶活性为指标,进行了土壤中菲、芘单一及复合胁迫响应研究。结果初步表明,菲、芘胁迫引起植物体内解毒代谢和抗氧化防御酶反应。菲、芘单一胁迫浓度为1mg kg-1时对细胞色素P450产生显著诱导;4 mg kg-1时P450酶含量明显被抑制,表明低浓度菲、芘单一胁迫对植物代谢解毒系统产生损伤;而菲、芘复合1mg kg-1时P450酶含量明显被抑制,说明菲、芘复合胁迫对植物的代谢解毒具有协同毒性效应。土壤中菲、芘单一胁迫未引起SOD酶活性的明显改变,复合胁迫下SOD酶活性出现微弱下降;菲、芘单一胁迫对CAT和POD酶活性具有显著抑制作用;复合胁迫对CAT产生抑制作用,而POD酶活性并未对菲、芘复合产生增强毒性响应。研究从代谢解毒和抗氧化防御酶系统两方面,为土壤低浓度PAHs污染诊断提供了实验依据。
Abstract:
Wheat (Triticum aestivum) was chosen to analyze the effect of two polycyclic aromatic hydrocarbons (PAHs), Phenanthrene (PHE) and Pyrene (PY) in brown meadow soil at low concentrations. The effects of PHE and PY were determined by analyzing the changes in activity of Cytochrome P450 (CytP450) and antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT). Results indicated that both PHE and PY caused changes in activity of CytP450 and the antioxidant enzymes, SOD, POD and CAT. CytP450 activity was significantly stimulated with 1 mg kg-1 of both PHE and PY individually and significantly inhibited with 4 mg kg-1, which showed that pollution stress of PHE or PY can damage the metabolism and detoxification systems of plants. Moreover, as PHE and PY combined at 1 mg kg-1, CytP450 was increased significantly more than when PHE and PY were applied individually, which illustrates obvious synergistic effects. No significant variation were found in activity of SOD in response to individual exposure of PHE or PY in soil, but SOD activity decreased slightly in response to a combined PHE and PY exposure. Great decrease variation was found in CAT and POD activity in response to individual exposure of PHE or PY in soil. No enhanced toxic effects were shown by POD in response to a PHE and PY combined exposure, however CAT showed increased inhibition. From the aspects of metabolism and detoxification as well as antioxidant enzyme activity, our study has provided experimental basis for the pollution diagnosis of PAHs in soils at low concentrations.
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