The ecological footprint model on the basis of sub-national hectare was presented in this paper according to the ecological footprint concept and model on the basis of global hectare or national hectare. The ecological footprint and its composition of each city in Zhejiang Province were evaluated and analyzed by using the modified model and the actual yield per unit area of Zhejiang in 2005.The results were compared with those which were evaluated in global hectare or national hectare method, and the differences were analyzed among them. When using sub-national hectare as the evaluation criterion, the ecological footprint, the ecological capacity and the ecological deficit of Zhejiang Province in 2005 would be 1.18s-nhm2/cap, 0.24s-nhm2/cap and 0.94s-nhm2/cap respectively, and the ecological pressure index was 4.91. The results suggest that Zhejiang Province is a representative area that has a large ecological deficit and high ecological pressure. They also suggested that there was a significant spatial difference according to the ecological footprint and its composition among the cities of Zhejiang. Compared with the average level of city ecological footprint in Zhejiang Province, Lishui, Quzhou, Huzhou and Jiaxing were in low ecological pressure states, and Ningbo, Hangzhou, Taizhou, Jinhua and Shaoxing were in middle ecological pressure states, while Zhoushan and Wenzhou were in high ecological pressure states. In the end, by applying IPAT, more eco-footprint demand of 104yuan GDP was found in Hangzhou, Jinhua, Jiaxing, Huzhou and Quzhou, which have a higher level than the average of Zhejiang, and suggestions were proposed that the level of local economic development must be considered to promote resource use efficiency.