Ozone (O3) is considered one of the important air pollutants in the present world. With the development of urbanization and the continuous increasing of atmospheric O3 concentration, more and more attention is focused on the impact of elevated O3 on plant. The impact of elevated O3 on trees is quite complicated. Based on the results of our experiments on the relationship between the trees and climate change in recent years, and related literature investigation, we will review the research advances in the impact of elevated O3 on eco-physiology of trees, such as the O3-induced visible injury, the impact of O3 on the growth and morphological structure, photosynthesis, nutrition metabolism, antioxidant system of trees and the impact of O3 in combination with other environmental factors, such as temperature, drought and elevated CO2. We hope these will provide a scientific reference for the further research on the impact of O3 on trees, especially on the sustainable forest management and the selection of urban tree species in respect to the increased atmosphere O3 concentration under the global climate change.