At the booting, flowering, and filling stages, an ASD Fieldspec HandHeld spectrometer was used to measure hyperspectral reflectance, red-edge parameters and their corresponding ecophysiological parameters of the winter wheat (Triticum aestivum) under different levels of drought stress. Chlorophyll content peaked at the flowering stage while leaf water content and leaf area index peaked at the booting stage. Chlorophyll content, leaf water content and leaf area index all decreased with increasing levels of drought stress. The first derivative spectra were used to determine the wavelength of the red-edge, the results showed that the red edge was at 728-730nm at the booting and flowering stages, then shifted to 734nm at the filling stage. Chlorophyll content was positively correlated with Dλ730∶Dλ702 and Dλ730∶Dλ718; LWC was positively correlated with Dλred and Dλ718; LAI was positively correlated with Dλ718, Dλred, and Sred; correlation coefficients were all greater than 0.5 (p＜0.05). Chlorophyll content and Dλ730∶Dλ702, LWC and Dλred were respectively linearly correlated (R2=0.87), while LAI and Sred were quadratically correlated (R2=0.68).