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万盼,胡艳波,张弓乔,王宏翔,李录林,王鹏,惠刚盈.甘肃小陇山油松与柴胡栽培土壤细菌群落特征.生态学报,2018,(17).http://dx.doi.org/10.5846/stxb201710251914  
甘肃小陇山油松与柴胡栽培土壤细菌群落特征
Soil bacterial communities under two Land Use Types of cultivated land in the piedmont region
投稿时间:2017-10-25  修订日期:2018-04-03
DOI: 10.5846/stxb201710251914
关键词土壤细菌  高通量测序  土壤化学性质  小陇山
Key WordsSoil  bacterial , High-throughput  sequencing, Soil  chemical properties, Xiaolongshan  Mountain
基金项目“十三•五”国家重点研发计划(2016YFD0600203)
作者单位E-mail
万盼 中国林业科学研究院林业研究所 wp7413841@163.com 
胡艳波 中国林业科学研究院林业研究所  
张弓乔 中国林业科学研究院林业研究所  
王宏翔 中国林业科学研究院林业研究所  
李录林 小陇山林业科学研究所  
王鹏 小陇山林业科学研究所  
惠刚盈 中国林业科学研究院林业研究所 hui@caf.ac.cn 
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摘要:
土壤细菌能够有效的促进土壤中物质循环和能量流动,其群落组成、数量及多样性能够反映土壤质量状况。研究不同土地方式下植被对土壤细菌群落结构和多样性的影响,有助于深入了解土壤健康状况,对实现的植被管理和土地可续利用具有重要意义。本文以甘肃小陇山移栽后4年生的油松大苗(A)和柴胡(B)植被为对象,采用第二代高通量测序技术对2种人工植被下土壤进行高通量测序,分析土壤细菌的Alpha多样性、群落组成和丰富度,并研究了土壤化学性质对细菌群落的影响。结果表明:1)两种不同植被的土壤细菌群落丰度和多样性无明显差异,但油松地土壤的细菌群落均匀度明显小于柴胡地土壤。2)所测土壤样品中共检测到细菌的37个门,84个纲,168个目;其中,主要的优势菌门为变形菌门(Proteobacteria),酸杆菌门(Acidobacteria),绿弯菌门(Chloroflexi),浮霉菌门( Planctomycetes),放线菌门(Actinobacteria),且油松地土壤中的变形菌门(Proteobacteria)的丰富度显著低于柴胡地土壤(P<0.05),绿弯菌门(Chloroflexi)丰富度却显著高于柴胡地(P<0.05);主要的优势菌纲为酸杆菌纲(Acidobacteria),β-变形菌纲(Betaproteobacteria),放线菌纲(Actinobacteria),变形菌纲(Alphaproteobacteria),芽单胞菌纲(Gemmatimonadetes ),但各优势菌纲的丰度在两个植被土壤之间差异不显著(P>0.05)。3)变形菌门(Proteobacteria)丰富度与土壤的碱解氮含量呈显著负相关(P<0.05),酸杆菌门(Acidobacteria)丰富度与TN显著负相关(P<0.01);通过RDA分析发现,碱解氮和全氮是影响细菌群落的最主要的土壤因子。可见,油松和柴胡植被土壤细菌群落组成及多样性差异不大,但油松地土壤细菌群落的物种分配较柴胡地集中,且优势细菌门中的变形菌门和绿弯菌门在两者之间的差异显著,同时,碱解氮和全氮含量是影响2种植被土壤细菌群落的主要因子。这些结论可为了解小陇山不同植被土壤微生物群落组成及多样性提供理论,也为当地山麓区域耕地进行土地合理利用和土壤科学管理提供科学参考。
Abstract:
Soil bacterial can effectively promote energy flow and material circulation in soil, its community composits, quantity and diversity can reflect the changing process of soil quality condition. The study aimed to explore the effects of different vegetation types on soil bacterial community structure and diversity and deeply understand soil quality condition for providing a scientific support for helping to achieve management and sustainable utilization of different vegetation. Soil was collected from the Seedling of Pinus tabulaeformis plantation and Bupleurum chinense in Xiaolongshan. Mountain of Gansu province. The High-throughput sequencing technology was used to analyze the alpha diversity, community composition and abundant of soil bacterial and fungus under two vegetation, and study the effects of soil chemical properties on soil bacterial community structure. 1) Abundance of bacterial community were not difference between two vegetation soil, however, bacterial community in Pinus tabulaeformis plantation soil was lower uniform degree than in Bupleurum chinense soil. 2) The soil in two vegetation all were detected with 37 bacterial phylums, 84 classes and 168 orders, the dominant phyla were Proteobacteria, Acidobacteria, Chloroflexi, Planctomycetes, and Actinobacteria, and Proteobacteria of soil in Pinus tabulaeformis was lower abundance than soil in Bupleurum chinense(P<0.05), Chloroflexi of soil in Pinus tabulaeformis was higher relative abundance in than soil in Bupleurum chinense(P<0.05), whereas the relative abundance of other dominant bacteria was not significant difference between two vegetation. At the class level, Acidobacteria, β-proteobateria, Actinobacteria, Gemmatimonadetes were predominant, the relative abundance of them was not significant difference between two vegetation. 3)Soil AN was negativly significantly correlated with the relative of Proteobacteria(P<0.05), soil TN was negativly significantly correlated with the relative of Acidobacteria(P<0.01). Results of RDA showed that AN and TN have the greatest impact on soil bacterial community. Soil bacterial community composition and diversity were not significant difference between two vegetation, the species of bacterial community in Pinus tabulaeformis soil was more centralized distributed than in Bupleurum chinense soil. Proteobacteria and Chloroflexi of the dominant phyla have significantly different in two vegetations, and AN and TN are main factors affecting the soil bacterial community. These study results of this paper is to provide the important theoretical for understand the diversity, community composition of soil microbial under different vegetation, and provide a scientific support for planting and soil management of Pinus tabulaeformis Carr. in Xiaolongshan Mountain.
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