首页关于本刊影响因子及获奖投稿须知订阅及广告专辑与专题学术会议绿色发表通道优秀论文 
吴永波,叶波.高温干旱复合胁迫对构树幼苗抗氧化酶活性和活性氧代谢的影响.生态学报,2016,36(2):403~410 本文二维码信息
二维码(扫一下试试看!)
高温干旱复合胁迫对构树幼苗抗氧化酶活性和活性氧代谢的影响
Effects of combined elevated temperature and drought stress on anti-oxidative enzyme activities and reactive oxygen species metabolism of Broussonetia papyrifera seedlings
投稿时间:2014-09-20  修订日期:2015-06-08
DOI: 10.5846/stxb201409201862
关键词高温  干旱  抗氧化酶活性  活性氧代谢  构树幼苗
Key Wordselevated temperature  drought  anti-oxidative enzyme activities  ROS metabolism  Broussonetia papyrifera seedlings
基金项目江苏省自然科学基金项目(BK2012819);江苏省高校自然科学研究重大项目(15KJA180003);江苏省生物学优势学科建设项目
作者单位E-mail
吴永波 南京林业大学, 江苏省南方现代林业协同创新中心, 南京 210037 yongbowu0920@163.com 
叶波 南京林业大学, 江苏省南方现代林业协同创新中心, 南京 210037  
摘要点击次数 1436
全文下载次数 2020
摘要:
近年来,全球气温不断升高,亚热带部分地区夏季高温和临时性干旱现象日益显著,高温与干旱严重威胁着植物的生存与生长。采用盆栽和人工气候室方式模拟不同的温度和土壤水分梯度,研究了高温与干旱复合胁迫对构树幼苗超氧化物歧化酶(SOD)、过氧化物酶(POD)与过氧化氢酶(CAT)活性、活性氧代谢和丙二醛(MDA)含量的影响。结果表明:(1)高温或干旱单一胁迫下,构树幼苗SOD、POD、CAT活性增加,复合胁迫下,SOD和POD酶活性高于单一胁迫,且随着复合胁迫时间延长而升高。SOD活性受温度和土壤水分双因素影响极其显著,复合胁迫对SOD活性有一定程度的叠加效应;(2)复合胁迫下,活性氧代谢物与MDA含量均显著高于单一胁迫,表明复合胁迫加剧对植物的伤害。通过改变抗氧化酶活性以减轻膜脂过氧化的伤害作用是有限的。
Abstract:
In recent years, under the global climate change scenario, the occurrence of seasonal high temperature and temporal drought phenomena has increased in some subtropical regions. Consequently, the survival and growth of plants are restrained under the combined stress of elevated temperature and drought. In the present study, a pot experiment was performed at the Xiashu Forest Station of Nanjing Forestry University in 2013. One-year-old Broussonetia papyrifera seedlings were grown in 27-cm pots with brown loam soil, using a completely randomized design with four replicates. Seedlings were initially grown in the field environment and then transferred to a controlled greenhouse with three elevated-temperature and soil-moisture treatments, and the effects of combined elevated temperature and drought stress on anti-oxidative enzyme activities and reactive oxygen species(ROS) metabolism were measured in B.papyrifera seedling leaves. The results indicated that under either elevated temperature or drought stress, the superoxide dismutase(SOD), peroxidase(POD), and catalase(CAT) activities of seedlings increased with the increase of stress level. Under the combined elevated temperature and drought stress, the activities of SOD and POD were higher than those under single temperature or drought stress, and increased over stress exposure time. Significant variation was observed in SOD activity of the seedlings between combined stress treatments, revealing the synergistic effects of combined stress on SOD activities. Under the combined elevated temperature and drought stress, ROS, including O2- production rates and hydrogen peroxide(H2O2) and malondialdehyde(MDA) contents, were significantly higher than those under the single temperature or drought stress treatment, which suggests the limited role of antioxidant enzyme activities in alleviating the damage of the cell membrane of seedlings due to differences in ROS metabolite accumulation.
HTML 查看全文   查看/发表评论  下载PDF阅读器

您是本站第 83167008 位访问者

Copyright © 2005-2019   京ICP备06018880号
地址:北京海淀区双清路18号
  邮编:100085    电话:010-62941099
  E-mail : shengtaixuebao@rcees.ac.cn
本系统由北京勤云科技发展有限公司提供技术支持