The spatial variability and distribution patterns of total nitrogen content in 0-100 cm deep soil were analyzed to determine the dominant factors affecting the spatial variability of total nitrogen content in different soil depths in Aohan County. The analyses were based on soil survey data, geostatistics, and canonical correspondence analysis. The results showed that the variation range of total nitrogen content was 0.43——0.68 g/kg in 0-100 cm soil depths, and the total nitrogen content decreased gradually with increasing soil depth. Obvious differences between North and South were found. A moderate spatial correlation of total nitrogen was found in 0-100 cm soil depths. With increasing soil depth, the spatial variability caused by random factors gradually decreased, and the spatial autocorrelation distance of total nitrogen decreased gradually. The effects of environmental factors on soil total nitrogen content from high to low were as follows:elevation > normalized difference vegetation index(NDVI) > clay content > soil bulk density > slope, in which soil clay and NDVI had higher correlations with surface soil, and elevation had a more significant effect on soil total nitrogen below 60 cm soil depths.