Conflict between human and wildlife has become a common problem in Qilian Mountain National Park. It is of great importance that we understand the attitude of the herdsmen to the causing of wildlife in the national park, to explore the law of causing of wildlife, to formulate prevention and control measures against the causing of wildlife, to protect the authenticity of the ecological system of the national park, and to promote the harmonious coexistence of herdsmen and wildlife. In this study, we investigated the conflict between herders and wildlife in the Qinghai area of Qilian Mountain National Park from 2014 to 2016 and their attitude towards wildlife. We found that November to March is the peak season for wildlife hunting in Qilian county; wolves and snow leopards are the main prey. Wolves are regarded as the most dangerous animals by the herdsmen. However, the actual data shows that the occurrence of snow leopards is higher than that of wolves. Compared with Qilian county, Tianjun county has a higher frequency of brown bears than wolves. The herders believe that the population of wolves and brown bears should be greatly controlled, because brown bears not only prey on livestock, but also harm the herders and damage their houses, threatening their lives. Snow leopards need further protection. Herders generally choose to use sheepdogs and intensive kennels to prevent wild animals from preying on livestock. Sheep are the main economic income of herdsmen in Qinghai area of Qilian Mountain National Park. Herdsmen cannot tolerate wolves eating their sheep, brown bears' direct predatory attacks are not frequent at present, but they need to be prevented in the future.