Myotis altarium is a typical cave-dwelling and quasi-endemic bat species (Vespertilionidae Chiroptera) in China. We investigated the roostpoint distribution, roosting behavior, body temperature, roost-site temperature, and the safety of the bats in the karst caves of Xiangxi Tujia and Miao Nationality Autonomous Prefecture, in the Hunan province, in recent years. On the basis of a total of 361 individual-by-times observed, the results indicated that their roost-points were distributed relatively, gathered within a 200 m distance of the cave tunnels from the entrances, and usually 2-6 m from the ground. Approximately 74% individual-by-times took on an upside-down roosting-posture with their abdomens clinging to the cave wall. The range of body temperature in the years was 3.57-20.25℃. Their body temperature was always slightly higher than the temperature of roost-sites, but there were no significant differences between them (P > 0.05). However, there were significant differences in body temperature and roost-site temperature in different months (P < 0.05). By a comprehensive evaluation of the roosting safety of these bats, we identified 60.1% individual-by-times as "high safety", whereas 22.7% and 17.2% were "medium safety" and "low safety", respectively. To conserve vulnerable species, it would be beneficial to strengthen biodiversity and cave biology education, which can enhance the conservation awareness and practices of bat species and cave resources for local people.